Functional genetic analysis of the leucinostatin biosynthesis transcription regulator lcsL in Purpureocillium lilacinum using CRISPR-Cas9 technology.
Identifieur interne : 000504 ( Main/Exploration ); précédent : 000503; suivant : 000505Functional genetic analysis of the leucinostatin biosynthesis transcription regulator lcsL in Purpureocillium lilacinum using CRISPR-Cas9 technology.
Auteurs : Yang Jiao [République populaire de Chine] ; Yan Li [République populaire de Chine] ; Yanlin Li [République populaire de Chine] ; Hongyi Cao [République populaire de Chine] ; Zhenchuan Mao [République populaire de Chine] ; Jian Ling [République populaire de Chine] ; Yuhong Yang [République populaire de Chine] ; Bingyan Xie [République populaire de Chine]Source :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2019.
Descripteurs français
- KwdFr :
- Clustered regularly interspaced short palindromic repeats (MeSH), Délétion de gène (MeSH), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Famille multigénique (MeSH), Hypocreales (génétique), Hypocreales (métabolisme), Peptides (métabolisme), Peptides antimicrobiens cationiques (MeSH), Phytophthora infestans (croissance et développement), Phytophthora infestans (effets des médicaments et des substances chimiques), Protéine-9 associée à CRISPR (MeSH), Recombinaison homologue (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Techniques de knock-out de gènes (MeSH), Transcription génétique (MeSH), Voies de biosynthèse (génétique).
- MESH :
- croissance et développement : Phytophthora infestans.
- effets des médicaments et des substances chimiques : Phytophthora infestans.
- génétique : Facteurs de transcription, Hypocreales, Voies de biosynthèse.
- métabolisme : Facteurs de transcription, Hypocreales, Peptides.
- Clustered regularly interspaced short palindromic repeats, Délétion de gène, Famille multigénique, Peptides antimicrobiens cationiques, Protéine-9 associée à CRISPR, Recombinaison homologue, Régulation de l'expression des gènes fongiques, Techniques de knock-out de gènes, Transcription génétique.
English descriptors
- KwdEn :
- Antimicrobial Cationic Peptides (MeSH), Biosynthetic Pathways (genetics), CRISPR-Associated Protein 9 (MeSH), Clustered Regularly Interspaced Short Palindromic Repeats (MeSH), Gene Deletion (MeSH), Gene Expression Regulation, Fungal (MeSH), Gene Knockout Techniques (MeSH), Homologous Recombination (MeSH), Hypocreales (genetics), Hypocreales (metabolism), Multigene Family (MeSH), Peptides (metabolism), Phytophthora infestans (drug effects), Phytophthora infestans (growth & development), Transcription Factors (genetics), Transcription Factors (metabolism), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : Transcription Factors.
- chemical , metabolism : Peptides, Transcription Factors.
- chemical : Antimicrobial Cationic Peptides, CRISPR-Associated Protein 9.
- drug effects : Phytophthora infestans.
- genetics : Biosynthetic Pathways, Hypocreales.
- growth & development : Phytophthora infestans.
- metabolism : Hypocreales.
- Clustered Regularly Interspaced Short Palindromic Repeats, Gene Deletion, Gene Expression Regulation, Fungal, Gene Knockout Techniques, Homologous Recombination, Multigene Family, Transcription, Genetic.
Abstract
Purpureocillium lilacinum is a promising commercial agent for controlling plant-parasitic nematodes and plant pathogens. Leucinostatins are a family of lipopeptides produced by P. lilacinum that are synthesized, modified, and regulated by a gene cluster consisting of 20 genes. Sequence analyses have indicated that lcsL, a gene in the lcs cluster, is a putative bZIP transcription factor. In this study, the CRISPR-Cas9 system was introduced to increase the efficiency of homologous recombination for the disruption of lcsL. The expression of genes in the cluster was significantly reduced in lcsL disruption mutants, and the output of leucinostatins was decreased to undetectable levels. In the lcsL overexpression strain, the expression of genes in the cluster and the yield of leucinostatins were all increased. The antagonism of both the wild type and mutant against Phytophthora infestans was also consistent with the gene expression and the output of leucinostatins. These results indicate that the gene lcsL is crucial for the regulating the synthesis of leucinostatins.
DOI: 10.1007/s00253-019-09945-2
PubMed: 31175427
Affiliations:
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Le document en format XML
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Jiao</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081</wicri:regionArea><wicri:noRegion>100081</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081</wicri:regionArea><wicri:noRegion>100081</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yanlin" sort="Li, 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Cao</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081</wicri:regionArea><wicri:noRegion>100081</wicri:noRegion></affiliation></author><author><name sortKey="Mao, Zhenchuan" sort="Mao, Zhenchuan" uniqKey="Mao Z" first="Zhenchuan" last="Mao">Zhenchuan Mao</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081</wicri:regionArea><wicri:noRegion>100081</wicri:noRegion></affiliation></author><author><name 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(MeSH)</term><term>Biosynthetic Pathways (genetics)</term><term>CRISPR-Associated Protein 9 (MeSH)</term><term>Clustered Regularly Interspaced Short Palindromic Repeats (MeSH)</term><term>Gene Deletion (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Gene Knockout Techniques (MeSH)</term><term>Homologous Recombination (MeSH)</term><term>Hypocreales (genetics)</term><term>Hypocreales (metabolism)</term><term>Multigene Family (MeSH)</term><term>Peptides (metabolism)</term><term>Phytophthora infestans (drug effects)</term><term>Phytophthora infestans (growth & development)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term><term>Transcription, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Clustered regularly interspaced short palindromic repeats (MeSH)</term><term>Délétion de gène (MeSH)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (métabolisme)</term><term>Famille multigénique (MeSH)</term><term>Hypocreales (génétique)</term><term>Hypocreales (métabolisme)</term><term>Peptides (métabolisme)</term><term>Peptides antimicrobiens cationiques (MeSH)</term><term>Phytophthora infestans (croissance et développement)</term><term>Phytophthora infestans (effets des médicaments et des substances chimiques)</term><term>Protéine-9 associée à CRISPR (MeSH)</term><term>Recombinaison homologue (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Techniques de knock-out de gènes (MeSH)</term><term>Transcription génétique (MeSH)</term><term>Voies de biosynthèse (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptides</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antimicrobial Cationic Peptides</term><term>CRISPR-Associated Protein 9</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Biosynthetic Pathways</term><term>Hypocreales</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term><term>Hypocreales</term><term>Voies de biosynthèse</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hypocreales</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de transcription</term><term>Hypocreales</term><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Clustered Regularly Interspaced Short Palindromic Repeats</term><term>Gene Deletion</term><term>Gene Expression Regulation, Fungal</term><term>Gene Knockout Techniques</term><term>Homologous Recombination</term><term>Multigene Family</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clustered regularly interspaced short palindromic repeats</term><term>Délétion de gène</term><term>Famille multigénique</term><term>Peptides antimicrobiens cationiques</term><term>Protéine-9 associée à CRISPR</term><term>Recombinaison homologue</term><term>Régulation de l'expression des gènes fongiques</term><term>Techniques de knock-out de gènes</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Purpureocillium lilacinum is a promising commercial agent for controlling plant-parasitic nematodes and plant pathogens. Leucinostatins are a family of lipopeptides produced by P. lilacinum that are synthesized, modified, and regulated by a gene cluster consisting of 20 genes. Sequence analyses have indicated that lcsL, a gene in the lcs cluster, is a putative bZIP transcription factor. In this study, the CRISPR-Cas9 system was introduced to increase the efficiency of homologous recombination for the disruption of lcsL. The expression of genes in the cluster was significantly reduced in lcsL disruption mutants, and the output of leucinostatins was decreased to undetectable levels. In the lcsL overexpression strain, the expression of genes in the cluster and the yield of leucinostatins were all increased. The antagonism of both the wild type and mutant against Phytophthora infestans was also consistent with the gene expression and the output of leucinostatins. These results indicate that the gene lcsL is crucial for the regulating the synthesis of leucinostatins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31175427</PMID><DateCompleted><Year>2019</Year><Month>11</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>103</Volume><Issue>15</Issue><PubDate><Year>2019</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Functional genetic analysis of the leucinostatin biosynthesis transcription regulator lcsL in Purpureocillium lilacinum using CRISPR-Cas9 technology.</ArticleTitle><Pagination><MedlinePgn>6187-6194</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-019-09945-2</ELocationID><Abstract><AbstractText>Purpureocillium lilacinum is a promising commercial agent for controlling plant-parasitic nematodes and plant pathogens. Leucinostatins are a family of lipopeptides produced by P. lilacinum that are synthesized, modified, and regulated by a gene cluster consisting of 20 genes. Sequence analyses have indicated that lcsL, a gene in the lcs cluster, is a putative bZIP transcription factor. In this study, the CRISPR-Cas9 system was introduced to increase the efficiency of homologous recombination for the disruption of lcsL. The expression of genes in the cluster was significantly reduced in lcsL disruption mutants, and the output of leucinostatins was decreased to undetectable levels. In the lcsL overexpression strain, the expression of genes in the cluster and the yield of leucinostatins were all increased. The antagonism of both the wild type and mutant against Phytophthora infestans was also consistent with the gene expression and the output of leucinostatins. These results indicate that the gene lcsL is crucial for the regulating the synthesis of leucinostatins.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jiao</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yanlin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticultural and Landscape, Hunan Agricultural University, Changsha, 410128, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Hongyi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mao</LastName><ForeName>Zhenchuan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ling</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yuhong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Bingyan</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. xiebingyan@caas.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2017YFD0200600</GrantID><Agency>Key Technologies Research and Development Program</Agency><Country></Country></Grant><Grant><GrantID>2018YFD0201200</GrantID><Agency>Key Technologies Research and Development Program</Agency><Country></Country></Grant><Grant><GrantID>Y2017JC28</GrantID><Agency>Central Public-interest Scientific Institution Basal Research Fund (CN)</Agency><Country></Country></Grant><Grant><GrantID>31772214</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>CARS-25</GrantID><Agency>Agriculture Research System of China</Agency><Country></Country></Grant><Grant><GrantID>CAAS-ASTIP-IVFCAAS</GrantID><Agency>Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D023181">Antimicrobial Cationic Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>76600-38-9</RegistryNumber><NameOfSubstance UI="C008268">leucinostatin A</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.-</RegistryNumber><NameOfSubstance UI="D000076987">CRISPR-Associated Protein 9</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D023181" MajorTopicYN="N">Antimicrobial Cationic Peptides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053898" MajorTopicYN="N">Biosynthetic Pathways</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000076987" MajorTopicYN="N">CRISPR-Associated Protein 9</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D064112" MajorTopicYN="N">Clustered Regularly Interspaced Short Palindromic Repeats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017353" MajorTopicYN="N">Gene Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="Y">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055786" MajorTopicYN="N">Gene Knockout Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059765" MajorTopicYN="N">Homologous Recombination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006999" MajorTopicYN="N">Hypocreales</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="Y">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="Y">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CRISPR-Cas9</Keyword><Keyword MajorTopicYN="N">Purpureocillium lilacinum</Keyword><Keyword MajorTopicYN="N">Secondary metabolisms</Keyword><Keyword MajorTopicYN="N">Transcriptional regulation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>05</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31175427</ArticleId><ArticleId IdType="doi">10.1007/s00253-019-09945-2</ArticleId><ArticleId IdType="pii">10.1007/s00253-019-09945-2</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Jiao, Yang" sort="Jiao, Yang" uniqKey="Jiao Y" first="Yang" last="Jiao">Yang Jiao</name></noRegion><name sortKey="Cao, Hongyi" sort="Cao, Hongyi" uniqKey="Cao H" first="Hongyi" last="Cao">Hongyi Cao</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Li, Yanlin" sort="Li, Yanlin" uniqKey="Li Y" first="Yanlin" last="Li">Yanlin Li</name><name sortKey="Li, Yanlin" sort="Li, Yanlin" uniqKey="Li Y" first="Yanlin" last="Li">Yanlin Li</name><name sortKey="Ling, Jian" sort="Ling, Jian" uniqKey="Ling J" first="Jian" last="Ling">Jian Ling</name><name sortKey="Mao, Zhenchuan" sort="Mao, Zhenchuan" uniqKey="Mao Z" first="Zhenchuan" last="Mao">Zhenchuan Mao</name><name sortKey="Xie, Bingyan" sort="Xie, Bingyan" uniqKey="Xie B" first="Bingyan" last="Xie">Bingyan Xie</name><name sortKey="Yang, Yuhong" sort="Yang, Yuhong" uniqKey="Yang Y" first="Yuhong" last="Yang">Yuhong Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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